The broadcaster shot his scenes for the Channel 4 soap earlier today (December 17), working alongside regular cast member Rachel Shenton, who plays glamour girl Mitzeee Minniver.
Shenton and the Hollyoaks crew travelled to Sky News's base in London for the scenes, which seemingly see Mitzeee appear on Holmes's Sunrise programme.
Mitzeee's semi-celebrity status makes her no stranger to TV appearances, but details of why she is hitting the airwaves this time around are being kept under wraps for now.
Writing on Twitter, Holmes joked: "Just to say @RachelShenton, thank you for being a delight to work with. Do you think the audience will take to our blossoming romance plot?"
He added: "Big thanks to Hollyoaks for giving young acting talent like myself and @RachelShenton our big breaks. Valentine's Day Special."
Meanwhile, Shenton kept fans guessing about the storyline by tweeting: "What is mitzeee doing with @eamonnholmes?"
Hollyoaks will air the episode in question next year.