The late-night E4 spinoff show - which is currently in pre-production at the Liverpool soap's home at Lime Pictures - will this year be headed up by Hollyoaks' new producer Paul Marquess.
Speaking about the project, Marquess said: "This commission is a huge vote of confidence in Hollyoaks from Channel 4. Seamlessly integrated with the 6.30pm shows, Hollyoaks Later 2010 will be the most exciting series so far. Prepare to be blown away."
Storyline details have yet to be confirmed but regular characters slated to be involved include husband and wife duo Mercedes (Jennifer Metcalfe) and Malachy Fisher (Glen Wallace), as well as McQueen cousins Michaela (Hollie-Jay Bowes) and Theresa (Jorgie Porter).
Newcomers Carl, Riley and Seth Costello - played by Paul Opacic, Rob Norbury and Miles Higson - will also appear alongside Rachel Shenton, who portrays new wannabe glamour model Mitzeee.
Commissioning editor for Channel 4 Roberto Troni said: "Hollyoaks is a hit with E4 audiences and we always look for opportunities to extend the brand and give fans something extra.
"The Hollyoaks Later spinoff provides a unique opportunity to experiment in a late night slot while staying true to the series. The late-night storylines will be a cocktail of fun, glamour and grown-up drama."
The five hour-long episodes are due to begin filming toward the middle of the year and are expected to transmit on E4 across consecutive weeknights in October.
Last year's run, which was headed up by EastEnders exec and former Hollyoaks boss Bryan Kirkwood, featured soapland's first ever parachute death and a special cameo appearance by Welsh songstress Bonnie Tyler.