The 24-year-old - who is the daughter of Echo and the Bunnymen star Ian McCulloch - will next week feature in a gritty storyline when she appears as new character Sami on the late-night soap spinoff.
Sami is the girlfriend of Michael Bisping's character Nathan - a bad boy who is about to cause trouble for Costello brothers Riley and Seth.
Speaking to the Liverpool Echo about Sami, McCulloch explained: "When we first see her, I think she seems quite manipulative, but I don't think she's bad at all - at least I hope not.
"I think she's probably quite scared of Nathan and does as she's told."
McCulloch enrolled in the Manchester School of Acting in 2008 after deciding to pursue a career on the screen. Hollyoaks Later was her first major acting job.
Reflecting on the experience, she added: "To be honest, I was nowhere near as nervous as I thought I'd be. It was so much fun and everyone was so nice, I just really enjoyed it. It was a fantastic opportunity for me and I learned so much from it in such a short space of time - it was just invaluable."
Hollyoaks Later airs at 9pm on E4 all next week.