Jennifer Metcalfe and Nicole Barber-Lane, who play feisty McQueen family members Mercedes and Myra, are among the cast who recently flew out to the Spanish island to film special scenes for the programme.
Digital Spy understands that the Ibiza storyline will feature in the fourth series of Hollyoaks Later, which airs for five nights on E4 later this year.
During shooting for the late-night spinoff show, Metcalfe was spotted wearing a fake baby bump on a beach, sparking speculation that Mercedes will fall pregnant in a forthcoming storyline twist.
Mercedes's current plot on the main soap has seen her pretend to be expecting a baby as part of a desperate bid to keep her steamy affair with Carl Costello under wraps. She has been engaged to Carl's son Riley (Rob Norbury) for several months.
As a result of the love cheat's deception, show bosses are keeping tight-lipped over whether Mercedes is still pretending to be pregnant in Hollyoaks Later or is expecting a baby with Riley for real.
A Hollyoaks source told Digital Spy this morning: "There's plenty of twists to come in this storyline and in a week of episodes airing at the end of the month, viewers will see Mercedes's big dilemma over Riley and the 'pregnancy' come to a head."
Hollyoaks Later will return to screens in the autumn.
> Hollyoaks star: 'Mercedes could cheat again'
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