The actress's character Donna Jackson will bow out of the BBC medical drama next month following a seven-year stint on screen. Her exit storyline sees her move away with love interest Kieran Callaghan (Barry Sloane).
Speaking to the Central Somerset Gazette about leaving Holby, Jacobs explained: "I'm definitely going to miss all the drama. Filming my final scenes was really overwhelming - it was incredibly emotional and intense, and it made it all very hard and raw.
"I knew it was the last few scenes she'd have and I put myself under a lot of pressure to get them right. But the cast and crew were all so supportive and they knew I wanted to put in the best performance I could.
"It wasn't until later that I realised I'd put myself under a hideously stressful situation and that I should just be enjoying the experience. Afterwards, I realised how much I was looking forward to embracing something new.
"I've been playing Donna for years, and it was a great chance to embrace something new and put myself in a new situation where I could play new characters."
Jacobs took on a theatre role after filming her final Holby scenes and she is now looking forward to new projects.
She said: "At the moment I've got the flu, so I'm going to recover from that and then start auditioning again. I want to do a wide variety of stuff - and I'm just going to see what comes along that takes my interest."
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