The scene, which took place in an August 31 edition of the soap, saw a fight erupt amongst the Queen Vic football team after they were told they had been kicked out of the local pub league.
The scene lasted for nearly two minutes and featured furniture being thrown, glasses being smashed and the pub being left in total disarray.
Broadcast reports that Ofcom received a single complaint about the episode, with a viewer saying it was too violent for the timeslot.
The media regulator will now assess whether the level of violence was appropriate for the 8pm broadcast time.
The single complaint is in marked contrast to the record number of complaints received by Ofcom for the EastEnders baby swap storyline, which a number of viewers found offensive in its depiction of a mother who reacted to the cot death of her own baby by stealing another child.
Ofcom ruled in favour of the BBC on that occasion, saying "sufficient editorial context" had been provided.
Watch the EastEnders pub brawl below: