The actor, who plays Marcus Dent on the ITV1 soap, took part in a reading relay organised by the Royal National Institute of Blind People (RNIB) as he paid a visit to Waterstone's in Piccadilly on Thursday morning.
Condou only intended to purchase a book but, after hearing details of the event being held inside, he agreed to get involved and later walked out as part of a successful Guinness World Records team.
The relay saw a grand total of 317 people reading aloud - one sentence at a time - from an exclusive Anthony Horowitz story called Double Eagle Has Landed, which has never been published in the UK.
Many blind and partially sighted people took part, reading Braille and giant print. The record attempt marked the launch of Read for RNIB Day, which raises awareness of reading services for blind and partially sighted people.
The event beat the previous Guinness World Record, held by Austria for 290 people in a reading relay.
Gaz Deaves, from Guinness World Records, commented: "What's wonderful about this record is the way it has unified people in reading. I was very impressed with how people used giant print and Braille as well as standard print. It was a fantastic achievement, well done RNIB and Waterstone's."
RNIB's chief executive Lesley-Anne Alexander added: "This is a fantastic result, and not only is RNIB now a Guinness World Records holder, it has been great to hold an event which is open to everyone of all levels of sight and reading abilities.
"But we shouldn't forget that just 7% of all books are available in fully accessible formats, so I urge everyone to get involved with Read for RNIB Day, and help us provide more reading solutions for blind and partially sighted people."
Condou returned to his Coronation Street role earlier this year.
> Charlie Condou: 'I'm staying in Coronation Street'
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