Coronation Street relocates to its new home at Salford Quays next year, leaving the future of its existing base at Quay Street uncertain.
As MOSI is adjacent to Coronation Street's current set, reports have often suggested that the two attractions could be combined.
However, such a move has now been ruled out by MOSI's director Jean M. Franczyk.
Franczyk told Manchester Confidential: "There's going to be someone out there who is probably the best in the world at running a Coronation Street visitor attraction, but it's not going to be the Museum of Science and Industry in Manchester.
"That's not our role. We have so much work to do in celebrating the historic site and contents we already possess. Coronation Street is for someone else."
Earlier this year, it was rumoured that the Coronation Street set could be listed by English Heritage. The full-street outdoor set was built in 1982.
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