The ban was included on a new list of rules for the shooting of the dangerous scenes which show factory boss Liam Connor (Rob James-Collier) dropping off a 300ft cliff.
The dramatic footage will also show Liam's girlfriend, Maria Sutherland (Samia Smith), falling into a river.
As a result the cast have been told that they must always have "both hands free" to keep themselves balanced on location. Cast and crew must also make sure they do not wander off the set paths and hard hats must be worn.
A source revealed the male cast members found the no urinating rule humorous but that they will take the new rules on board. The insider said: "The rules are unprecedented but even those who joked about the no-peeing one have accepted them.
"It's just that Cumbria will be freezing - so we'll be more susceptible to calls of nature."