The BBC's commercial body already offers a free online game for the Anne Robinson-fronted programme, but there will now be a charge for players take part, reports The Guardian.
Worldwide has licensed Amuso.com to launch the pay-to-play game, which has in turn signed up The Weakest Link co-founder Fintan Coyle as a strategic advisor.
Amuso.com will charge players a variety of amounts to play the game, starting from around £1 per session. Worldwide will not take any revenue directly from players.
To prevent illegal gambling, the corporation has introduced an online protection system to stop under-18s from taking part. If anyone makes a deposit of more than £50 the game also asks them to provide identification via a passport or driving licence.
Licensing the Weakest Link game is believed to be part of Worldwide's strategy to unlock fresh revenue streams as it prepares to sell off its lucrative magazines division.
Last month, BBC director general Mark Thompson revealed that the organisation earned record profits of around £140m in the year to March 31, up from £103m in the previous 12 months. However, Worldwide has targeted annual profits of over £200m by 2012.
"BBC Worldwide is always keen to extend a brand beyond the traditional use, and social gaming is one of great interest to us," said Worldwide's executive vice president of digital entertainment Robert Nashak.
Yesterday, the BBC announced that the first episode of its Doctor Who game has been downloaded over 500,000 times in its first 12 days of availability.