As of 5.59am this morning, Sky Real Lives and Sky Real Lives 2 ceased broadcasting on the digital satellite platform. Sky Real Lives HD and Sky Real Lives +1 have also shut down.
Launched three years ago to replace Sky Travel, Sky Real Lives produced a range of real-life programmes aimed at women in the 35 to 54 age range.
However, Sky has decided that the channel's audience can be "better served" by allocating the programming budget to the main Sky1 and Sky2 channels.
"By focusing content investment on those channels which offer greater reach and exposure, we believe we can better meet customer demands," said head of Sky Real Lives Barbara Gibbon.
"There will be no compromise on screen, nor in satisfying Sky Real Lives viewers, who'll continue to enjoy a rich diversity of high-quality pay-TV programming."
Speculation is already mounting on Digital Spy's Sky forum about what will happen with the vacant slots on Sky's programming guide.
Possible options include the expected launch of Living HD, after Sky recently acquired the Living channel operator Virgin Media Television.