The advert, entitled the 'Next Big Thing', features consumers queuing outside an Apple store waiting for the launch of the new iPhone 4S handset.
The Apple 'fanboys' then see other people outside the queue going about their daily business and using the Samsung Galaxy S II, marvelling at the features of the Google Android-powered handset, including the larger screen and the 4G connectivity.
After being shown the smartphone, one man says: "I could never get a Samsung. I'm creative."
To which, his friend replies: "Dude, you're a barista."
The advert has received a mixed reaction already online, with some people hitting out at Samsung's tactics, and others criticising the firm for suggesting that baristas are not creative.
However, some people have pointed to Apple's Get A Mac adverts featuring David Mitchell and Robert Webb that poked fun at PCs, as evidence that Apple is in no position to complain.
Speaking to Business Insider, Samsung's chief marketing officer Todd Pendleton said: "Just from initial reactions, even Apple owners and lovers are talking about this in a positive way.
"We're seeing positive quotes from people out there who may not be willing to buy our product."
Brian Wallace, Samsung's vice president of strategic marketing, added: "We're not targeting fanboys. We're not going to convert them.
"We're using them as a foil to target current Android users upgrading to a new phone and people entering the smartphone market for the first time."
Samsung and Apple are locked in an increasingly bitter battle for control over the smartphone market, including various patent disputes around the world.
When the iPhone 4S launched in Australia last month, Samsung set up a temporary shop near Apple's store in Sydney offering the Galaxy S II smartphones for just AUS$2, in a bid to tempt away iPhone customers.
> Samsung's Apple dispute to be heard by Australian court in March
> Samsung modifies Galaxy handsets to avoid Dutch sales ban
Watch Samsung's 'Next Big Thing' ad below: