Walter Isaacson's book claimed first place at the expense of Tina Fey's Bossypants despite launching as late as October.
Amazon did not reveal specific sales figures, but its senior editor of books Chris Schluep described them as "phenomenal" in both print and digital.
"After the year of recommending books to our customers, it's always fun to see what books really resonated with them," he said.
"We chose Steve Jobs as one of the Top 10 best books of the year, and even though it was published in October, the sales have been phenomenal in both formats."
Steve Jobs was published by Simon & Schuster. Sony has already claimed the rights to adapt the book for cinema. Social Network writer Aaron Sorkin is said to be in talks to pen a screenplay for the project.
Amazon also revealed that two of its top ten titles - The Mill River Recluse by Darcie Chan and The Abbey by Chris Culver - were exclusive to its Kindle Store, and unavailable in print.
Schluep added: "We're really excited that Kindle Direct Publishing authors have taken two of the top spots this year for book sales overall."
Steve Jobs is available on Amazon's US store for $17.50, or £10.77 through its UK counterpart.
The 2011 Amazon bestsellers list in full is as follows:
1.Steve Jobs by Walter Isaacson
2. Bossypants by Tina Fey
3. A Stolen Life by Jaycee Dugard
4. The Mill River Recluse by Darcie Chan
5. In the Garden of the Beasts by Erik Larson
6. A Dance with Dragons by George R.R. Martin
7. The Paris Wife by Paula McLain
8. The Litigators by John Grisham
9. The Abbey by Chris Culver
10. Inheritance (The Inheritance Cycle) by Christopher Paolini