The collaboration, first announced in January of this year, sees a range of 3D content from Eurosport becoming available via SpotPass to view on a dedicated Eurosport video application on the 3DS. The player will be offered for free from the Nintendo eShop.
The videos, launching weekly from today, are two to three-minute 3D clips from Eurosport's popular WATTS programme, featuring a mix of sporting action and funny outtakes from around the world. Users will be able to download the videos for free.
Nintendo Europe managing director of marketing and PR Laurent Fischer said: "We are always striving to bring fun and exciting new content to our fans and this new partnership with Eurosport will do just that.
"We are looking forward to launching the WATTS videos each week along with a host of other 3D content on Nintendo Video from our various partners."
Heather Bowler, the global communications director at Eurosport, added: "The 3D clips from WATTS, one of Eurosport's most established programmes, are guaranteed to engage and entertain Nintendo 3DS users.
"Eurosport and Nintendo are both leaders within their industries so there is a natural brand fit with this collaboration, which will extend our reach within an important target audience."
The Eurosport partnership is part of Nintendo's drive to bring more 3D video entertainment to the 3DS, which launched in March but had a price cut in the summer following sluggish sales.
Nintendo Video is also working with the Berliner Philharmoniker Orchestra, Red Bull Media, Sky and Aardman Animations to bring content to its SpotPass service, allowing 3DS owners to access 3D videos over the console's web connection.
> Nintendo 3DS to receive Red Bull video content