The watchdog revealed that the ISP attracts 0.56 complaints per 1,000 customers, the majority of which were about line faults and service issues.
BT was next on the list with 0.38 complaints per 1,000 customers, while Sky and Virgin Media garnered the least complaints with 0.15 and 0.17 complaints per 1,000 customers respectively.
Although TalkTalk has topped Ofcom's customer dissatisfaction table for some time, its ratio of complaints appears to be on the slide, down from 0.61 during the previous quarter.
Ofcom measured customer satisfaction in the Pay TV market for the first time, reporting that BT Vision received the most negative feedback, while Sky boasted the highest level of satisfaction.
However, the volume of complaints against BT in this sector showed signs of decline from Q4 2011 to Q1 2012, falling from 0.38 to 0.27 complaints per 1,000 users. Sky and Virgin Media remained steady on 0.3 and 0.8/0.7 respectively.