John Lewis, the first firm outside the US to partner with book chain Barnes & Noble, will stock the Nook Simple Touch and Nook Simple Touch with GlowLight in its 37 UK stores and at www.johnlewis.com from October.
As previously announced, Barnes & Noble will also offer the devices at its own UK online retail store, going head to head with Amazon's market-leading Kindle.
The eReaders will be backed up by Barnes & Noble's digital book and content store.
"John Lewis is where knowledgeable customers turn for trusted advice on the best products to purchase, and they are a perfect partner to help launch Nook in the UK," said Jamie Iannone, the president of digital products at Barnes & Noble.
"We look forward to bringing more choice and convenience to discerning UK customers with our critically acclaimed line of reading devices and expansive selection of content."
The Nook Simple Touch is a lightweight device that features a WiFi connection and E Ink technology for reading even in bright sunlight. GlowLight is patent-pending lighting technology for comfortable reading while in the dark.
John Lewis buying director for electricals Ed Connolly said: "This award-winning line of eReaders adds greater choice to this expanding category.
"We think our customers will love Nook's adaptability thanks to its E Ink display and unique built-in GlowLight, perfect for day or night-time reading."
Further information on Nook pricing and exact availability will be announced in the autumn, said Barnes & Noble.
Commercial terms of the John Lewis partnership were not disclosed.