Commuters can purchase unlimited access for £2 per day, £5 a week or £15 per month when the free service comes to an end in early 2013.
It was previously reported that EE and Vodafone users will still be able to take advantage of free internet on the tube after the service providers entered an agreement with Virgin.
Subscribers to Virgin's TV, broadband and mobile packages will also receive the London underground service for free, leaving O2 and 3 customers in the UK capital out in the cold.
WiFi is currently available in 72 tube stations with a further 20 adding connectivity in December and an additional 28 in early 2013.
The service was originally introduced as a free trial to coincide with the Olympic Games, but its popularity convinced Virgin to make it a permanent offering.