The now-Facebook-owned photo-sharing app this week disabled the integration of Twitter cards, which has changed the way Instagram pictures are displayed on the microblogging website.
Essentially, this means images are now poorly cropped rather than being nicely displayed on Twitter timelines as they were before.
Users now have to visit the actual Instagram feed or the new instagram.com profiles to see the photos as they were meant to be seen.
In an official statement, Twitter said: "Users are experiencing issues with viewing Instagram photos on Twitter. Issues include cropped images.
"This is due to Instagram disabling its Twitter cards integration, and as a result, photos are being displayed using a pre-cards experience. So, when users click on tweets with an Instagram link, photos appear cropped."
The move has been widely viewed by the media as the first salvo in a feud between Twitter and Instagram, after the latter was bought by Facebook for around $730 million this year.
Instagram has instead acted first, but its founder and chief executive Kevin Systrom denied that this was part of a feud with Twitter.
Speaking yesterday at the LeWeb'12 event, Systrom said that the change is part of "an evolution of where we want links to our content to go".
He said that even though Twitter integration will always remain in some shape or form, the relationship between the two sites is changing, particularly online.
"We're working on building an awesome web presence, which we just launched," said Systrom.
"We revamped our web properties, and now we're able to staff up teams to work on web properties with the Facebook acquisition."
He added: "We've launched several improvements to our website that allow users to directly engage with Instagram content through likes, comments, hashtags, and now we believe the best experience is for us to link back to where the content lives."
Systrom said that the media always wants to portray such changes as 'tit-for-tat' battles between tech giants, but he is more focused on Instragram's growth as a service.
He also reminded people that Twitter co-founder Jack Dorsey was one of the photo app's earliest investors, although there have been reports that Dorsey was upset Instagram plumped for Facebook rather than Twitter.