
The show was watched by 1.1m (7.7%) from 6pm, a fall of 400,000 on last week's opening episode.
Don't Forget the Lyrics, another gameshow introduced on Sky One last week, dipped from 946,000 (6.1%) to 663,000 (3.8%) in the 7pm slot.
Despite the audience fall, Gladiators was comfortably the night's most watched show on a digital-only channel.
One of the only programmes to come close was a repeat of Britain's Got More Talent, which drew 936,000 (4.6%) on ITV2 from 9pm.
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