Members of the cast of EastEnders will join Les Dennis, Christopher Biggins, Nicky Chapman, Robert Webb, Dick and Dom and a number of celebrity chefs to re-create famous dance moves from popstars and films in the series premiere.
The show will feature a live performance from The Saturdays, who will sing their Comic Relief single 'Just Can't Get Enough'.
The second heat will feature Jo Brand, the cast of Hollyoaks, Blue Peter presenters, Keith Lemon, Paddy McGuinness, Neil Fox, Nancy Sorrell and the stars of Dragons' Den.
Dancers in all three heats will be assessed by head panellist Anton Du Beke, who will be accompanied by two guest judges.
The panel and public will combine to decide which competitors make it through to the dance-off on Red Nose Day weekend.
Let's Dance For Comic Relief starts on Saturday, 21 February on BBC One.