Steve Jones and Claudia Winkleman will return to host the programme, which will run for four weeks on BBC One.
Comics Katy Brand, Rufus Hound and Shappi Khorsandi will be joined on the dance show by sports stars Peter Shilton, Rodney Marsh, Willie Thorne and Dennis Taylor.
Jenny Eclair, Linda Robson, Lesley Jospeh and Susie Blake will also compete in the charity event.
The rotating judging panel will be made of comedians such as Frank Skinner and Jack Dee, as well as former contestants.
Dance routines on this year's show will include a Beyoncé 'Single Ladies' performance and versions of 'Grease Lightning' and 'Smooth Criminal'. Peep Show's Robert Webb won the 2009 series with a Flashdance-inspired routine.
"Saturday nights on the BBC are all about eventful, big, fun, family entertainment and Let's Dance For Sport Relief absolutely fits that bill," said BBC comedy entertainment executive editor Jo Wallace.
Let's Dance For Comic Relief returns on Saturday, February 20 on BBC One.