It was confirmed in April that comedian Michael McIntyre will host the 82nd annual event at the London Palladium next month.
Take That will perform two songs at the event, while X Factor judge Cole will perform her new single 'The Flood'.
In a press release, McIntyre said: "I am unbelievably excited to be hosting The Royal Variety Performance this year at the London Palladium in its centenary year. It is a dream come true to be introducing the likes of Take That, Cheryl Cole and Prince Charles's own personal booking, N-Dubz."
Other acts confirmed to appear include Britain's Got Talent winners Spelbound, tenor Russell Watson, Dancing On Ice professional skater Daniel Whiston and comedians John Bishop and Jack Whitehall.
The 2010 Royal Variety Performance will be broadcast on BBC One in December.