As previously revealed, the event will see the comic take on the roles of host, team captain and panellist alongside the rotating special guests, while paying tribute to a range of popular panel shows.
Highlights include Walliams and his mother taking on Miranda Hart and her on-screen mother Patricia Hodge in The Generation Game and Paul O'Grady hosting Blankety Blank as Walliams competes against Barbara Windsor, Stacey Solomon and David Tennant.
The Come Fly With Me star will also join Russell Tovey, Sue Perkins and Sean Lock will be joined by Stephen Fry for QI, while Celebrity Juice will see Leigh Francis test Walliams, Lauren Laverne, Germaine Greer, Rufus Hound and Jedward.
Jack Dee, Claudia Winkleman, Jason Manford, David Mitchell, Dara O'Briain, Sue Perkins, Jo Brand, Jimmy Carr, Lee Mack, Richard Bacon, Vernon Kay, Sir David Frost, Charlie Brooker, Christopher Biggins and Ulrika Jonsson are among the many famous faces who will join the broadcast.
Other shows which will feature in the challenge include Through The Keyhole, Room 101, Mock The Week and It's Only TV.
24 Hour Panel People will stream live online from midday on Saturday March 5. Five 30-minute highlight programmes will air on BBC Three in the run up to Red Nose Day.