The programme - hosted by David Mitchell, Jimmy Carr, Charlie Brooker and Lauren Laverne - began in January after the success of the presenters' previous Alternative Election Night special.
The show includes a mixture of sketches, interviews and comment on current events. The final instalment in the series aired last week.
Channel 4 has now denied speculation that the programme will not be returning and explained that the future of the show is still up in the air.
In a message on the Channel 4 press Twitter page, the broadcaster said: "Contrary to the rumour mill - 10 O'Clock Live has not been cancelled. No decision has been made."
In March, Channel 4's comedy and entertainment commissioner Darren Smith defended the show's ratings, describing them as "fantastic".