The Born This Way singer will be interviewed tomorrow night (October 6) by the presenter, ahead of the show's broadcast on ITV1 this weekend.
The 25-year-old, who has previously been interviewed by Ross on his BBC chatshow, will also perform new single 'Yoü and I'.
Comedian Lee Evans and chef Jamie Oliver will also appear on the programme, which last week featured Alan Partridge.
The Jonathan Ross Show continues this Saturday at 10pm on ITV1.
GaGa recently revealed that she has started work on her next studio album.
Watch Lady GaGa's appearance on Friday Night with Jonathan Ross below: