ITV recently confirmed that the duo - who defected from the BBC last year - would leave the struggling breakfast show to focus on their peak-time projects.
Ahead of hosting alongside Chiles for the final time this morning, Bleakley tweeted: "Morning all, what lovely messages this morning for my last 3am wake up all (sic)"
"As some of you will know, this is our last show," Chiles told viewers. "As a leaving present, I'm taking my funny animal clips clipboard with me! We would just like to say thank you to the rest of the on-air team." The Northern Irish presenter added: "And especially to all of our production staff."
Chiles continued: "I'm off to concentrate on the football and Christine is being put on ice so to speak. Good luck have a great Christmas. Bye for now," while Bleakley concluded: "That is just about it from us, good luck, have a great Christmas. Bye bye."
Tony Blackburn and Chris Tarrant were among the guests on the duo's final edition of Daybreak.
Following Holly Willoughby's exit, Bleakley will take over as co-host of Dancing on Ice, while her former One Show colleague focuses on his other projects for the broadcaster.
Speaking last week, Bleakley admitted that she was "looking forward to having my life back again", acknowledging that she "certainly won't miss 3am starts".
The new presenters of Daybreak have not yet been announced.
> Christine Bleakley 'has no ill-feelings about Daybreak axe'