Claudia Winkleman will host the charity show, which will feature British sports stars performing at top comedy venues to help raise money for Sport Relief.
Ex-England cricket captain Michael Vaughan, World Cup rugby star Ben Cohen and sports broadcaster Gabby Logan will all be taking part in the show. Other sports stars participating will be revealed in the coming weeks.
Comics Patrick Kielty, Jason Manford, Andi Osho, Chris Ramsey and Daniel Sloss will mentor the sports stars ahead of their big night at London's Bloomsbury Theatre, which will air on BBC Three in the build up to Sport Relief.
Vaughan commented: "I used to play cricket in front of 20,000 people but the thought of making 500 people laugh is terrifying.
"And having spent a day with my comedian, I've realised just how unfunny I am."
Host Winkleman added: "I am incredibly proud to be part of Stand Up for Sport Relief. It sounds both exciting and terrifying which is win-win.
"I'll try and support the brave sportsmen and women who are going to be comics for one night as much as possible."
> Pixie Lott, Tinchy Stryder to guest on 'Let's Dance for Sport Relief'
> Louie Spence for Sport Relief sock slide challenge
> Olly Murs trousers in Sport Relief training video - watch