Strictly Come Dancing's Craig Revel Horwood, DJ Trevor Nelson, Professor Marcus du Sautoy and comic Josie Lawrence have signed up for the three-part series, which will see them competing for the chance to perform at the Royal Opera House, Covent Garden.
The winning trainee will conduct an Act of a legendary opera with a cast of international young artists.
Each celebrity will have their own personal mentor to guide them through the pitfalls and high drama of the stage. The first programme will see them challenged on the basic building block of conducting an aria.
Operatic stars, conductor Sir Mark Elder, soprano Danielle de Niese and orchestral musician Dominic Seldis will judge the competitors' efforts.
Great British Bake Off presenter Sue Perkins triumphed in the first series in 2008, beating DJ Goldie in the finale.
Controller of BBC Two Janice Hadlow said: "Having pipped Goldie to the conductor's podium in series one, it's time for Sue Perkins to pass her champion's baton to a brand new set of personalities who face a very different challenge.
"I look forward to seeing whether their natural musical ability will be enough to help them master the skills needed in the new series, which will take viewers right to the heart of one of the world's greatest opera institutions."
Royal Opera House chief executive Tony Hall said: "It's a wonderful way of bringing opera to BBC audiences. It's going to be a fascinating journey watching the Maestros meet the challenges of opera in the thick of a working theatre and I wish each of them all the best!"
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