Despite a tabloid rumour earlier this week claiming the comedian had changed his mind, a telling picture of the TV Burp cast and crew posing in front of the audience for the last time has emerged on Twitter.
Ex-EastEnders star Cheryl Fergison appears in the shot, as well as TV Burp's version of the much-loved, now-deceased soap character.
Classic TV Burp icon and Big Brother 8 star Brian Belo is also visible in the front row, while recurring puppet Lord Sugar and monstrous wild child Wagbo are both tipped to make final cameos.
Digital Spy forum member ZoeMcCallister posted: "Definitely the last ever one. Just been in the audience. Harry said not to take notice of The Sun rumours.
"Without giving too much away, the final scene is epic for TV Burp diehards and is definite closure for the show. Also, probably the best episode of TV Burp in years. Harry's really put effort into the final one."
Jason100 added in the TV Burp thread: "I was there tonight and there were a few surprises. Not sure about anyone else who went but I felt a bit teary at the end."
> Harry Hill's TV Burp best moments - video
Back in January, Hill said that the satirical clip show would bow out with a "huge" finale.
Following his split with his agent from TV Burp producer Avalon, it was reported that Channel 4 had lined up a £2 million deal for a sketch show and film.
The 47-year-old former stand-up, who had rejected a deal with ITV worth a reported extra £1 million, is working on a new sports panel game with Alistair McGowan.
Watch Harry Hill's recent 'Toffee In My Shoe' routine on TV Burp below: