Don't Sit in the Front Row will see the deadpan Live at the Apollo comic chair a group of comedians creating comedy routines based on members of the studio audience.
Guest comedians will then have to dig into the lives of the front row audience members.
There will be four rounds, with one audience member and comic eliminated in each. The 'Golden Chair' prize will be awarded to the winner.
Created by former Have I Got News for You producers Giles Pilbrow and Mark Barrett, the 16-part series will be filmed later this year and broadcast in the autumn.
Sky Atlantic commissioner Antonia Hurford-Jones said: "The spontaneity of the audience interaction is what makes Don't Sit in the Front Row so exciting."
Pilbrow added: "The comics are shooting from the hip - and you can tell they are. There's a certain joy about genuine spontaneity which can't be faked."
Sky Atlantic's most recent entertainment format, Ann Widdecombe's Cleverdicks, has so far flopped in the ratings.
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