The comedy panel show, which features Holly Willoughby and Fearne Cotton, will be hoping to follow in the footsteps of fellow ITV2 hit and last year's YouTube Award winner The Only Way Is Essex and take home the gong at this year's Arqiva British Academy Television Awards.
Benedict Cumberbatch's Sherlock, Sir David Attenborough's documentary series Frozen Planet and Mary Berry's hit cookery competition series The Great British Bake Off are also battling for the prize.
Completing the shortlist, which was devised by a team of journalists and broadcasters including Digital Spy, are Channel 4's Educating Essex and Sam Bain and Jesse Armstrong's university sitcom Fresh Meat.
[Sherlock - Fresh Meat]
Fans can vote for their favourite from their list between April 19 and 5pm on Thursday, May 24.
The TV BAFTAs will be hosted by Dara O'Briain at the Royal Festival Hall on Sunday, May 27, and will air on BBC One.
[Great British Bake Off - Frozen Planet]
"With the diverse range of nominated TV programmes this year, the YouTube community has its work cut out in selecting this year's Audience Award winner," said YouTube marketing manager Mike Sullivan.
Amanda Berry, chief executive of BAFTA, added: "It is terrific to see such a variety of genres represented by the nominated programmes for this year's YouTube Audience Award. Each programme has really engaged the television audience and earned its place on this year's shortlist."
Viewers can vote for their favourite show online at YouTube.com/baftaonline. The official Twitter hashtag is #YTAAvote.
The BAFTA YouTube Audience Award jury talk about the purpose of the gong: