Frank Skinner will return for a second run of the revamped version of the long-running BBC One series, according to Comedy.co.uk.
The show was originally hosted by Nick Hancock and then Paul Merton from 1994 to 2007, before it returned in January 2012 with Skinner as host.
However, the series saw a new format, where three guests per episode nominated items in different categories, rather than just one guest each show.
The eight-part 13th series will shoot in October, with a broadcast expected in early 2013 on BBC One.
Room 101's series finale in March dropped around 800,000 viewers from its launch show.
Watch a clip from Room 101 below: