Stefan Abingdon, Dru Wakely and Ashley Horne star as the fictionalised wannabe band hoping to become superstars.
Watch a behind-the-scenes clip from The Midnight Beast below:
The trio have established themselves a huge fanbase online with more than 45 million YouTube views to date.
Midnight Beast originally found mainstream fame when their parody of 'Tik Tok' by Ke$ha became a viral hit.
The sitcom features the boys' trademark songs, dance routines, music video, special guests and fictional exploits as they live together in an East London flat.
The Midnight Beast is released on DVD on August 13. The trio will perform at HMV Oxford Street and sign copies at 5.30pm on the same day.