The legendary broadcaster has been given access to The University of Liverpool's Vet School to host Rolf's Animal Clinic.
Harris famously hosted hit BBC One series Animal Hospital during the '90s.
His new Channel 5 series will "follow teams of highly-skilled professionals and students whose daily work involves dealing with medical emergencies and treating the injuries and ailments of a wide variety of animals - from domestic pets and farm livestock to exotic zoo creatures".
Speaking about his TV return, Harris said: "The welfare of animals is something that has always been close to my heart and it's wonderful to be presenting this series which covers the fascinating and skilled work of the vets dealing with such an amazing range of animals."
Commissioning editor Ian Dunkley added: "The series gets to the emotional heart of what it is to be a vet - from the drama of life-saving equine surgery, to how best to deal with an overweight pet. Having Rolf as the presenter is a fantastic addition to the series."
Watch the classic Animal Hospital opening titles below: