Six young people will ride from Llandudno to London to help raise funds for disadvantaged children and young people across the UK.
Host Matt Baker, who last year raised £1.9 million after completing the gruelling journey from Edinburgh to London, will ride alongside the group to offer support and advice. Alex Jones will also rally support along the way.
'Team Rickshaw' will be made up of six youngsters who have been or still are supported by BBC Children in Need funded projects.
They will start the journey on November 9 and will attempt to complete the 411-mile route in eight days.
Baker said: "The Rickshaw Challenge is one of the toughest challenges I've ever taken on. These youngsters are going to have their work cut out for them.
"That's why the public needs to get behind Team Rickshaw and come out and cheer them on. I know from the challenge last year what a difference the public's support can make.
"We need to get behind them as they strive to raise as much money as possible for BBC Children in Need."
Jones added: "I've met all the team. They are a really inspiring bunch. It will be a real honour for Matt and me to be with them on their journey."
Viewers can donate £5 to the cause by texting TEAM to 70705, with texts costing £5 plus one standard rate message. More details can be found on the Children in Need website.