The host also broke the Guinness World Record for 'Most Questions Asked on a TV Chat Show' as he presented from 7pm to the early hours of Friday evening.
Graham Norton's Big Chat for Comic Relief - pictures:
Guests on the show included Terry Wogan, Russell Tovey, Jimmy Carr, Martin Freeman, Keith Lemon, Louis Smith and Warwick Davis. There was music from Example, Paloma Faith, Hurts and Laura Mvula.
The show also introduced a Celebrity Kissing Booth that allowed members of the public to win a smooch with stars such as David Gandy and Abbey Clancy.
The Big Chat is available to watch on iPlayer and you can still donate at ComicRelief.com.