Ricky Gervais's The Office creation was shown in a video for the much-loved song, strolling down a multicultural street in black and white.
The full David Brent sketch looked back at what has happened to the character over the last ten years.
Now reinvented as a music producer, the 'chilled-out entertainer' cannot resist stealing the limelight from the young artists he is meant to be promoting.
Other Comic Relief highlights have included Jessie J having her head shaved and Simon Cowell marrying his ideal partner.
Viewers also saw David Tennant kissing John Bishop, the return of the Vicar of Dibley cast, and Will Ferrell starring in a special Comic Relief sketch as Anchorman's Ron Burgundy.
To donate to Comic Relief, go to the Red Nose Day website. You can also purchase Red Nose Day 2013: The Best Bits, Pt. 1 on iTunes.
Photo gallery - Comic Relief: Funny for Money in pictures: