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The Irish judge turned around to flash a V-sign at the crowd after his act John & Edward received a mixed response to their sing-off performance of Robbie Williams's 'Rock DJ'.
A spokesman for Walsh has now told The Sun: "Louis apologises if any offence was caused."
Walsh has complained about audiences booing the twins in previous weeks, branding those responsible disrespectful and "rude".
John & Edward survived another round of the competition last night after triumphing over Lucie Jones in the sing-off.
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