The boyband became the latest act to be eliminated after falling into the bottom two tonight (November 11) with fellow group Union J.
District3 performed a rendition of Bruno Mars's 'Just The Way You Are', while Union J sang Adele's 'Set Fire to the Rain'.
Mentor Louis Walsh was visibly emotional at seeing his remaining acts competing against one another in the sing off. Union J are now Walsh's final act in this year's competition.
After the two groups performed to earn a place in the final six, it was left to the judges to save their preferred act.
Walsh was the first judge to be asked for his vote but refused to choose between his acts. Gary Barlow expressed his surprise at District3's "worst vocal performance to date" and chose to send them home. Nicole Scherzinger also chose to send home the trio, meaning that they would be the act to leave the show.
Tulisa then revealed that if her vote had counted she would have saved District3, as she has felt closer to them as a group from the start of the competition.
The 'Young' singer had predicted on last night's show (November 10) that the groups may be splitting viewers, leaving them vulnerable to falling into the bottom two.
On The Xtra Factor, District3 admitted that whilst they are "absolutely gutted" to be leaving the show, they have had a "wicked time". They also wished Union J good luck in the rest of the series.
Member Greg West added: "We wish the boys all the luck in the world."
The result means that six acts remain on the ITV1 singing competition. Three of the acts left, Jahmene Douglas, Rylan Clark and James Arthur, belong to Nicole Scherzinger's category.
James Arthur topped this week's Digital Spy's poll for best singer after his rendition of Adele's 'Hometown Glory'.
The X Factor continues next Saturday (November 17) at 8pm.
Watch District3's audition performance of Boyz II Men's 'I'll Make Love to You' below: