Doo-wop group The TestostaTones were announced by Britain's Got More Talent host Stephen Mulhern as having received the most public votes of the 24 acts shortlisted in the online contest.
Their prize was to perform in front of judges Simon Cowell, Amanda Holden, Alesha Dixon and David Walliams at auditions in Birmingham live on YouTube.
They are the first act from the upcoming series to be unveiled and their audition represented the first footage of the new-look panel.
The five-piece a cappella band - made up of relatives and friends - started off with a performance of 1950s classic 'Get A Job' by The Silhouettes.
However, Cowell soon stopped their routine and asked if they "do different takes on [modern] songs".
The TestostaTones, who have been singing together for just 18 months, responded with a doo-wop version of 'Born To Be Wild' from Steppenwolf, but they were soon buzzed off by all four judges.
The fate of The TestostaTones will be revealed when Britain's Got Talent series six airs in March.
Watch the Britain's Got Talent team talk to Digital Spy below: