The self-proclaimed cougar received a higher share of the public vote than fellow nominees David, JJ and Sam, and left after 21 days in the compound.
As Davina read out Jo's name, the 41-year-old appeared unsurprised by the news and began to kiss and hug the housemates goodbye.
While standing at the top of the stairs waiting to exit the house, a shocked Jo spun around and shouted: "They're really booing me!"
She left the house to Tom Jones's 'What's New Pussycat?' and a largely negative reception.