Tens of thousands of you voted earlier this month, and while the top ten threw up some surprises, the winning moment was never really in doubt.
From the series two finale 'Doomsday', it's the moment that The Doctor bid goodbye to a tearful Rose (for the first time).
Grab some Kleenex, click here to relive the moment, then hit 'play' below to see what our panel of Who experts had to say:
And so that concludes our top ten countdown. Thankyou to everyone who voted, all of our lovely video contributors and of course, to DT himself for being so ruddy brilliant.
Did the right moment win? And do you agree with our panellists? Add your comments to this entry below!