The cartoon character is seen attending an audition for Springfield's Got Talent where he performs in front of Simon Cowell.
In a spoof of Boyle's rendition of 'I Dreamed A Dream' on the ITV1 show, he says: "My name is Homer Simpson, I'm 39 years old and, well, I've never been kissed.
"My dream is to be a great singer like Susan Boyle," he adds.
The clip, which has appeared online, will be included on the show's 20th anniversary season.
Boyle recently became the subject of ridicule in an episode of South Park, in which the character Ike complained about the Scottish star's exposure.
The 47-year-old attracted worldwide attention after her Britain's Got Talent audition was viewed by over 100 million people on YouTube.