The 47-year-old actress, who played Amanda Woodward on the 1990s series, will reprise her role on Tuesday, November 17.
"We're ecstatic to have the chance to bring Amanda Woodward back to Melrose Place," said executive producers Todd Slavkin & Darren Swimmer.
"Heather’s involvement in the show is something we've been working on for some time as we couldn't imagine creating and producing this show without the iconic character’s inclusion."
Locklear will join her former co-stars Laura Leighton, Thomas Calabro, Josie Bisset and Daphne Zuniga, who have all reprised their roles for the reboot.
Melrose, which airs Tuesdays on The CW, made a disappointing debut, averaging 2.27m for its first episode.