On his show The O'Reilly Factor, the presenter showed a clip of the cartoon which pokes fun at Fox News.
In the video, a Fox helicopter hovers near the Statue of Liberty with the motto: "Fox News: Not Racist But #1 With Racists" painted on the side.
The helicopter also begins to crash and the pilot shouts: "We're unbalanced! It's not fair!"
O'Reilly criticised the joke, saying: "Continuing to bite the hand that feeds part of it, Fox Broadcasting once again allows its cartoon characters to run wild... Pinheads? I believe so."
Earlier this month, The Simpsons was renewed for a 23rd season.