Aykroyd will appear on the legal drama as a judge, according to CBS's Twitter account.
"This Just In…DAN AYKROYD reunites w Blues Brothers partner JIM BELUSHI guesting on "THE DEFENDERS" in early 2011 as a judge needing help! (sic)" the network wrote.
The casting will reunite Aykroyd with his Blues Brothers touring partner Jim Belushi, who plays one of the lead defence attorneys on the show, reports The Hollywood Reporter.
Series co-creator Kevin Kennedy recently confessed that Aykroyd was on their "dream list" of possible guest stars.
The Defenders airs Wednesdays at 10pm on CBS.