Chicago-based Harpo Productions revealed that the final instalment of The Oprah Winfrey Show will air on May 25, reports The AP.
Winfrey's syndicated daytime chatshow premiered in 1986. It currently airs in 145 countries worldwide.
The TV host explained last month that she feels "energised" as she approaches the end of her talkshow's final season.
The 57-year-old is expected to turn her full attention to her OWN Network after The Oprah Winfrey Show ends.