The 'Born This Way' singer will make her first appearance on Kimmel's show since October 2008. She will speak to Kimmel before a live performance on the Bud Light Outdoor Stage in Hollywood.
Jimmy Kimmel Live will shut down the entire block behind the studio for the first time for GaGa's performance, which will include the late night debut of her latest single 'You and I'.
Coldplay, Jay-Z and Depeche Mode have previously shut down areas of Hollywood during their appearances on the programme.
Harrison Ford will also appear as a guest on Thursday night's edition, where he will promote his latest movie Cowboys and Aliens.
The film's director Jon Favreau was previously confirmed to be taking charge of Wednesday night's edition of Jimmy Kimmel Live.
Watch Lady GaGa perform 'Just Dance' on Jimmy Kimmel Live below: