The comedy, written by Sex and the City scribe Michael Patrick King and stand-up Whitney Cummings, focuses on two girls from very different economic backgrounds who end up working in the same diner in Brooklyn.
The duo, played by Kat Dennings and Beth Behr, become friends and decide to go into business together.
Channel 4's head of acquisitions Gill Hay said: "It is a rare thrill to find a new comedy that is effortlessly funny, warm-hearted and which has such a broad audience appeal.
"I am so delighted that Channel 4 has secured this show and we are all looking forward to introducing the UK to such a treat."
2 Broke Girls is Channel 4's latest US acquisition. The channel recently picked up the rights to Claire Danes's new drama Homeland.
Meanwhile, E4 will broadcast comedies New Girl and Apartment 23 as well as animations Napoleon Dynamite and Allen Gregory.
2 Broke Girls will premiere in the US on September 19 but Channel 4 has not yet announced a start date for the UK.
Watch an extended preview of 2 Broke Girls below: