The singer will sing his hit 'Red Solo Cup' for the very first time on broadcast television at the Las Vegas awards gala.
'Red Solo Cup' is the second single off Keith's 15th studio album Clancy's Tavern, which topped the US country album chart in October.
The American Country Awards will also feature performances from American Idol winner Scotty McCreery, Alabama, Pistol Annies, The Band Perry and Blake Shelton.
Jason Aldean and Zac Brown Band lead this year's ACA nominees with eight nods each, while Taylor Swift has received nominations in six categories.
The second annual American Country Awards, hosted by Trace Adkins and Kristin Chenoweth, air from the MGM Grand Garden Arena on December 5 at 8pm ET/PT on Fox.
Fans can vote for their favourites at www.theacas.com until Friday (December 2).
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Watch Toby Keith's unedited 'Red Solo Cup' music video below: